DNA Cloning for Bacterial Protein Production

Image from Khan Academy
  • Preparation of an insert. This is a small DNA segment that ultimately codes for a scientist’s desired protein.
  • Preparation of a plasmid vector. This is what will carry the insert DNA into the bacteria, while also providing a few other functions necessary for DNA cloning. [2]
  • Joining of the insert and plasmid vector in a process referred to as ligation. Once joined, the insert and plasmid vector pair is now called a recombinant plasmid.
  • Introducing the newly joined recombinant plasmid into the E. coli bacteria.
  • Confirming that successful E. coli clones have been made using techniques like colony PCR and DNA sequencing.
Image from Khan Academy
Image from Khan Academy
Image from Khan Academy

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Welcome to the Macromoltek blog! We're an Austin-based biotech firm focused on using computers to further the discovery and design of antibodies.

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Macromoltek, Inc.

Macromoltek, Inc.

Welcome to the Macromoltek blog! We're an Austin-based biotech firm focused on using computers to further the discovery and design of antibodies.

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